HELP DESK - Expanded for Personal Discussion with Voice and Whiteboard

Whiteboard for discusions at OPSD Help Desk.

Our HELP DESK has been expanded from simple Chat so we can now speak directly to visitors seeking help in sample preparation and preservation. 

To use this free service, simply click on the whiteboard above to enter Chat.  Ask to speak with one of our scientists, and if we are available, we will provide  you with a link to enter our HELP DESK room.  All discussions are private.  If necessary, we will schedule a convenient time when we can meet.

NEW!  Sample Homogenization Considerations for NextGen Sequencing

DNA sequencing has evolved significantly since the late 1970s, when shotgun cloned libraries were deciphered a couple hundred bases at a time using radioactive labels, hand poured gels, films, and a darkroom. As the demand for sequence data shifted from genes to genomes, or from kilobases to gigabases, gene cloning was excluded as the sophistication and throughput of DNA sequencing increased. In the sequencing lab, Escherichia coli lost its job.

The latest in Next Generation Sequencing (NGS) now allows for direct sequencing of individual DNA molecules or clonally amplified DNA. The primary systems used in NGS, manufactured by Pacific Biosciences and Illumina®, can generate gigabases to terabases of data per run (compared to megabases for the previous generation) by directly measuring bases incorporated during DNA polymerization. Gone are dideoxy terminators and pyrosequencing, replaced by fluorescent nucleotides that are detected during synthesis (i.e., SBS or sequencing by synthesis). Furthermore, the single-molecule real-time (SMRT™) technology of Pacific Biosciences measures ... (More)

50 ml Polycarbonate Vials

OPS Diagnostics is pleased to offer a new 50 ml polycarbonate grinding vial that has the same base format and design as the popular 15 ml PC vial, but over three times the volume. The increased size of this vial allows for the processing of up to 3 gm of plant tissue, 8 gm of seeds (22 corn kernels), and 4 gm of animal tissue. Vials are sealed with newly designed silicone lined caps.

We have added a guide "Selection of Grinding Vials for Sample Processing" to assist in vial selection. This guide provides recommendations regarding sample mass, grinding media, buffer volume, and homogenizer for designing efficient homogenization processes.

50 ml Polycarbonate Grinding Vial for Large Samples

Co-Isolation of Plant & Pathogen/Parasite DNA

The isolation of DNA from multiple divergent species is important for investigating microcosms, whether it is symbiotic biomes or host/parasite interactions. In the case of the latter, rapid and easy isolation of DNA from the host/pathogen can allow for detection and monitoring of infection. Within the agricultural realm, a simple method for isolating DNA from both the host and pathogen, followed by quantification, can demonstrate the presence of a pathogen and be used to determine the extent of the infection (Gachon and Saindrenan, 2004). For instance, the ability to rapidly assess such host/pathogen levels could be useful in determining the application of pesticides (McCartney et al., 2003).

OPS Diagnostics developed the Synergy™ DNA isolation chemistry in order to rapidly isolate plant DNA for standard PCR assays. Synergy™ is a chloroform-free chemistry that relies on bead beating to separate contaminates from the nucleic acids. (More)