Homogenization of nails (keratin) by bead beating

Keratin is a common protein of interest that is found in fingernails and toenails and can be released by homogenization. Keratin is a resilient structural protein resulting from the complex network formed by keratin monomers. It is the bundled keratin proteins and the layers of dead, flattened cells that gives fingernails and toenails their strength. However, fingernails can easily be homogenized using a high throughput homogenizer and a stainless steel grinding ball. A standard protocol for fingernail homogenization can be found below. The protocol was designed using Homo sapiens fingernails. Multiple samples may be homogenized using a 24 Well PC Vial Set.

Protocol for fingernail homogenization.

Weigh 20 mg of fingernail clippings and place into a 4 ml polycarbonate vial.
Insert a 3/8” stainless steel grinding ball and securely fasten the lid.
Homogenize at 1500 rpm for five minutes in a HT Homogenizer.

Results