R_xeSearch: An Educational Journey

Replacement Kit for Lesson #3

Price: $145

Lesson #3 of the R_xeSearch curriculum involves exploring a hypothetical enzyme assay found in a pathogenic microorganism.  The kit provides the replacement reagents for the basic assay and screening the chemical library.  Each kit includes the following materials:

1 Bottle of Substrate (60 ml of 50 mM lactic acid)

1 Bottle of Buffer (60 ml of 200 mM Tris, pH 8)

1 Bottle of Water (125 ml)

Chemical library compounds (20 ml in 12 small jars)

Two empty Diluted Enzyme tubes

Three vials of 1.5 ml lyophilized Enzyme Concentrate 

16 vials of 1.5 ml lyophilized PMS/INT/NAD

250 reaction tubes (tall 4 ml plastic tubes)

15 vials of water (2 ml) for student groups

250 empty reagent vials (2 ml) for student groups

330 Transfer pipettes (for lessons 1, 3, and 5)

420 + Labels for reagents, library and reactions tubes

R_xeSearch: An Educational Journey

Replacement Kit for Lesson #3

Examine the effects of pH, temperature, and inhibitors on enzymes.

Price: $145

Investigating Enzymes provides the materials for 15 student groups to run enzyme assays and examine the parameters that affect activity.  This kit uses lactate dehydrogenase which can be measured by the conversion of  lactic acid to pyruvate.  The NAD dependent reaction generates a red color that can be observed visually qualitatively or quantitatively using a spectrophotometer.  The kit comes with five buffers (pH 5-9) which are used to demonstrate the impact of pH on enzymes.  A set of inhibitors is also included.  Guidelines for temperature effects and temperature of inactivation are discussed in the teacher's guide.  Each kit includes:

1 Bottle of Substrate (60 ml of 50 mM lactic acid)

1 Bottle of Buffer (60 ml of 200 mM Tris, pH 8)

1 Set of buffers (pH 5, 6, 7, 9)

1 Bottle of Water (125 ml)

1 Set of inhibitors

Two empty Diluted Enzyme tubes

Four vials of 1.5 ml lyophilized Enzyme Concentrate 

16 vials of 1.5 ml lyophilized PMS/INT/NAD

250 reaction tubes (tall 4 ml plastic tubes)

15 vials of water (2 ml) for student groups

200 empty reagent vials (2 ml) for student groups

200 Transfer pipettes

Labels for reagents and reactions tubes

Teacher's Guide

Investigating Enzymes Kit