Pre-Filled Microfuge Disruption Tubes

Filling microfuge tubes with beads is a painful, time consuming task. Not any more! OPS Diagnostics now offers pre-filled tubes using our acid washed grinding media. Each tube contains 325 μl of acid washed beads in a 2 ml screw-cap microfuge tube. This allows for the homogenization of 650 μl sample, typically microorganisms, while allowing for the required headspace for efficient disruption by vortexing or bead beating.* Tubes are available with silica beads as well as the higher impacting zirconium beads.

Recommended size applications for Pre-Filled Tubes:

100 μm - Bacteria, sonication additive

200 μm - Bacteria, small yeasts (e.g., Pichia)

400 μm - Yeasts (Saccharomyces)

800 μm - Molds, pollen

Pre-Filled Tubes can be used with the Pulsing Vortex Mixer, GenoGrinder, or HT Homogenizer.

Product	Product Number	List Price
100 μm Silica Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 100-100-01	$110
100 μm Zirconium Beads, 600 mg Pre-Filled Microfuge Tubes (100/pkg)	PFAW 100-100-02	$110
100 μm Zirconium Beads, 1200 mg Pre-Filled Microfuge Tubes	PFAW 100-100-26	$110
200 μm Zirconium Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 200-100-03	$110
400 μm Silica Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 400-100-04	$110
400 μm Zirconium Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 400-100-30	$110
800 μm Silica Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 800-100-05	$110
800 μm Zirconium Beads, Pre-Filled Microfuge Tubes (100/pkg)	PFAW 800-100-29	$110
1.0 mm Zirconium Beads, Acid Washed Pre-Filled Tubes (100/pkg)	PFAW 1000-100-21	$110
1.4 mm Zirconium Beads, Acid Washed Pre-Filled Tubes (100/pkg)	PFAW 1400-100-19	$110
1.7 mm Zirconium Beads, Acid Washed Pre-Filled Tubes (50/pkg)	PFAW 1700-50-16	$56
2.8 mm Stainless Steel Grinding Balls, Pre-Filled Tubes (50/pkg)	PFSS 2800-50-20	$78
3.0 mm Zirconium Beads, Acid Washed Pre-Filled Tubes (50 pkg)	PFAW 3000-50-17	$56
500 μm Garnet and a 6mm Zirconium Grinding Satellite (100/pkg)	PFMM 500-100-25	$124
800 micron & 1.4 mm Zirconium Beads, Acid Washed Pre-filled Tubes (100/pkg)	PFMM 800-100-27	$124
100 μm Silica Beads, 1.4 mm Zirconium Beads and a 4 mm silica bead, Acid Washed Pre-filled Tubes (100/pkg)	PFMM 4000-100-28	$124

Also see Low Binding and Molecular Biology Grade Pre-Filled Tubes.

* Work in our laboratory determined that the most efficient homogenization of microbes by bead beating used 1 part beads to 2 parts culture broth. The total volume of the beads and broth should not exceed half the volume of the tube. Consequently we fill our tubes with 325 μl of beads so that following the addition of 650 μl sample, the total volume is just under half the volume of the tube.