Lyophilization Reagent (2X Concentrate)

This clear solution is designed to simplify protein lyophilization.  It is composed of a mixture of a matrix (caking agent) and lyoprotectant, that when mixed act synergistically to provide a solution with a high eutectic point, solid matrix, and excellent protein stability.  It is prepared as a 2X concentrate that can be used as is, or mixed in equal concentration with a sample.  Mixing the 2X Concentrate with buffers may require a reassessment of freeze drying parameters.  Lyophilization Reagent is sterile filtered and bottled aseptically. It is provided in 500 ml PET bottles.

Application Note: Evaluation of Lyophilization Reagent Concentrate for the Preservation of Lactate Dehydrogenase from Mouse Tissue Lysates

Freeze Drying Indicator

This colorimetric indicator reagent changes color during the freeze drying process giving the user a real-time measurement of dryness.  This formulation combines the protein stabilizers with an indicator dye.  It can be used in parallel with the standard reagent to show when freeze drying is completed.  This indicator reagent should be run along side of critical samples and not used as the lyoprotectant itself (protein lyophilization with the indicator should be discarded). This reagent is also useful for mapping and validating freeze dryers.  The reagent can be used as a visual tool for operational qualification of large and small freeze dryer units. It is filter sterilized and aseptically bottled in 500 ml PET bottles.

Microbial Freeze Drying Buffer - No Animal Proteins!

The Microbial Freeze Drying Buffer is evolved from a standard buffer described in the ATCC lyophilization manual and has currently been improved to be free of all animal products/proteins!  To use, cultured microorganisms are mixed with the buffer and simply freeze dried.  Viability of bacteria in this buffer is over 90%, far exceeding methods relying on sucrose and skim milk.  This product is sterile filtered and aseptically filled  in 500 ml PET bottles.